期刊
MECHANISMS OF AGEING AND DEVELOPMENT
卷 134, 期 7-8, 页码 321-330出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mad.2013.05.004
关键词
Snell dwarf mice; Sulfur metabolism; Redox; Sexual dimorphism; Global DNA methylation; Mathematical model
资金
- National Institutes of Health [HL58984, AG031736, AG013283]
Changes in sulfur-based redox metabolite profiles in multiple tissues of long-lived Snell dwarf mice were compared with age- and sex-matched controls. Plasma methionine and its oxidation products, hypotaurine and taurine, were increased in Snell dwarfs while cystine and glutathione levels were decreased, leading to an oxidative shift in the redox potential. Sexual dimorphism in renal cystathionine p-synthase (CBS) activity was observed in control mice but not in Snell dwarfs. Instead, female Snell mice exhibited similar to 2-fold higher CBS activity, comparable to levels seen in male Snell dwarf and in control mice. Taurine levels were significantly higher in kidney and brain of Snell dwarf versus control mice. Methionine adenosyltransferase (MAT) was higher in liver of Snell dwarfs, and the higher concentration of its product, S-adenosylmethionine, was correlated with elevated global DNA methylation status. Application of a mathematical model for methionine metabolism revealed that the metabolite perturbations in Snell dwarfs could be explained by decreased methionine transport, increased MAT and increased methyltransferase activity. Our study provides a comprehensive map of systemic differences in the sulfur network between Snell dwarfs and controls, providing the necessary foundation for assessment of nutrition-linked metabolic status in long-lived versus control animals. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据