期刊
MECHANISMS OF AGEING AND DEVELOPMENT
卷 133, 期 1, 页码 37-45出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mad.2011.12.002
关键词
Dietary restriction; Ageing; High resolution respirometry; PGC-1 alpha; Mitochondrial biogenesis
资金
- Biotechnology and Biological Sciences Research Council (BBSRC) [BB/H012850/1]
- College of Life Science and Medicine, University of Aberdeen
- Carnegie's Trust for the Universities of Scotland
- Biotechnology and Biological Sciences Research Council [BB/H012850/1] Funding Source: researchfish
- BBSRC [BB/H012850/1] Funding Source: UKRI
Dietary restriction (DR) is suggested to induce mitochondria] biogenesis, although recently this has been challenged. Here we determined the impact of 1, 9 and 18 months of 30% DR in male C57BL/6 mice on key mitochondrial factors and on mitochondrial function in skeletal muscle, relative to age-matched ad libitum (AL) controls. We examined proteins and mRNAs associated with mitochondrial biogenesis and measured mitochondrial respiration in permeabilised myofibres using high resolution respirometry. 30% DR, irrespective of duration, had no effect on citrate synthase activity. In contrast, total and nuclear protein levels of PGC-1 alpha, mRNA levels of several mitochondrial associated proteins (Pgc-1 alpha, Nrf1, Core 1, Cox IV, Atps) and cytochrome c oxidase content were increased in skeletal muscle of DR mice. Furthermore, a range of mitochondrial respiration rates were increased significantly by DR, with DR partially attenuating the age-related decline in respiration observed in AL controls. Therefore, DR did not increase mitochondrial content, as determined by citrate synthase, in mouse skeletal muscle. However, it did induce a PGC-1 alpha adaptive response and increased mitochondrial respiration. Thus, we suggest that a functionally 'efficient' mitochondrial electron transport chain may be a critical mechanism underlying DR, rather than any net increase in mitochondrial content per se. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
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