期刊
MECHANISMS OF AGEING AND DEVELOPMENT
卷 132, 期 1-2, 页码 43-54出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mad.2010.12.001
关键词
Dietary restriction; UCP1; Mitochondria; ROS; Insulin; Adenine nucleotide translocase
资金
- Biotechnology and Biology Research Council
- Unilever plc through BBSRC [BBS/S/N/2004/11555]
Restricted feeding regimes in rodents that extend longevity lower the rate of mitochondrial reactive oxygen species generation. This effect is not dependent upon the depression of the state III and IV mitochondrial respiration rates. Mitochondria from liver, skeletal muscle and brown adipose tissue adapt to DR feeding with a lowered membrane potential that results from an enhanced proton leak across the inner membrane. Mitochondria! ROS generation is very sensitive to the magnitude of the membrane potential and the enhanced proton leak and ROS generation rates are reversible by exogenous insulin in liver and heart mitochondria. The adenine nucleotide translocase (ANT) was shown to be the dominant proton leak channel induced under DR feeding regimes in these tissues, while in brown fat mitochondria, UCP1 was activated, but this was not sensitive to exogenous insulin treatment. The effect of DR to modify the proton leak, membrane potential and ROS generation rate can be simulated by a range of non-esterified free fatty acids (NEFA) acting on the ANT to enhance its protonophoric activity. Mobilisation of NEFA under DR feeding, when insulin plasma concentrations are extremely low, explains the indirect action of insulin to counteract the effects of DR feeding on mitochondrial ROS generation. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据