期刊
MECHANISMS OF AGEING AND DEVELOPMENT
卷 132, 期 10, 页码 459-467出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mad.2011.07.003
关键词
Tissue elasticity; Arteriosclerosis; Collagen; Elastic fibers; Scanning acoustic microscopy
资金
- British Heart Foundation [PG 07/099/23758, FS/08/036/25364]
- Research into Ageing [266]
- Wellcome Trust [WT085981AIA]
- British Heart Foundation [PG/07/099/23758, PG/09/062/27872] Funding Source: researchfish
Age-related loss of tissue elasticity is a common cause of human morbidity and arteriosclerosis (vascular stiffening) is associated with the development of both fatal strokes and heart failure. However, in the absence of appropriate micro-mechanical testing methodologies, multiple structural remodelling events have been proposed as the cause of arteriosclerosis. Therefore, using a model of ageing in female sheep aorta (young: <18 months, old: >8 years) we: (i) quantified age-related macro-mechanical stiffness, (ii) localised in situ micro-metre scale changes in acoustic wave speed (a measure of tissue stiffness) and (iii) characterised collagen and elastic fibre remodelling. With age, there was an increase in both macro-mechanical stiffness and mean microscopic wave speed (and hence stiffness; young wave speed: 1701 +/- 1 m s(-1), old wave speed: 1710 +/- 1 m s(-1), p < 0.001) which was localized to collagen fibril-rich regions located between large elastic lamellae. These micro-mechanical changes were associated with increases in both collagen and elastic fibre content (collagen tissue area, young: 31 +/- 2%, old: 40 +/- 4%, p < 0.05; elastic fibre tissue area, young: 55 +/- 3%, old: 69 +/- 4%, p < 0.001). Localised collagen fibrosis may therefore play a key role in mediating age-related arteriosclerosis. Furthermore, high frequency scanning acoustic microscopy is capable of co-localising micro-mechanical and micro-structural changes in ageing tissues. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
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