期刊
MEAT SCIENCE
卷 97, 期 4, 页码 404-409出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.meatsci.2014.03.011
关键词
Canine derivatives; Meatballs; Extensive autoclaving; Halal food industry
资金
- Universiti Malaya Research Grant [RG153-12AET]
- Malaysian Ministry of Higher Education MIS scholarship [KPT.B.600-18/3]
A polymerase chain reaction (PCR) assay for the assessment of dog meat adulteration in meatballs was developed. The assay selectively amplified a 100-bp region of canine mitochondrial cytochrome b gene from pure, raw, processed and mixed backgrounds. The specificity of the assay was tested against 11 animals and 3 plants species, commonly available for meatball formulation. The stability of the assay was proven under extensively autoclaving conditions that breakdown target DNA. A blind test from ready to eat chicken and beef meatballs showed that the assay can repeatedly detect 0.2% canine meat tissues under complex matrices using 0.04 ng of dog DNA extracted from differentially treated meatballs. The simplicity, stability and sensitivity of the assay suggested that it could be used in halal food industry for the authentication of canine derivatives in processed foods. (C) 2014 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据