期刊
MEAT SCIENCE
卷 98, 期 4, 页码 726-735出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.meatsci.2014.07.018
关键词
CE-TOF MS; Metabolomics; Muscle type; Myosin heavy chain isoform; Pork; Postmortem aging
资金
- Kieikai Research Foundation (Japan)
- Japan Society for the Promotion of Science [JSPS KAKENHI 23780276]
To determine key compounds and metabolic pathways associated with meat quality, we profiled metabolites in postmortem porcine longissimus lumborum (LL) and vastus intermedius (VI) muscles with different aging times by global metabolomics using capillary electrophoresis-time of flight mass spectrometry. Loading analyses of the principal component analysis showed that hydrophilic amino acids and beta-alanine-related compounds contributed to the muscle type positively and negatively, respectively, whereas glycolytic and ATP degradation products contributed to aging time. At 168 h postmortem, LL samples were characterized by abundance of combinations of amino acids, dipeptides, and glycolytic products, whereas the VI samples were characterized by abundance of both sulfur-containing compounds and amino acids. The AMP and inosine contents in the VI were approx. 10 times higher than those in the LL at 4 h postmortem, suggesting different rates of inosine 5'-monophosphate (IMP) accumulation by adenylate kinase 7 and 5'-nucleotidase, and subsequent different production levels of IMP and hypoxanthine between these two porcine muscles. (C) 2014 Elsevier Ltd. All rights reserved.
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