期刊
MEAT SCIENCE
卷 82, 期 2, 页码 252-259出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.meatsci.2009.01.023
关键词
Real-time PCR; SYBR-Green; Porcine DNA; 12S rRNA; 18S rRNA; Feedstuffs
资金
- Ministerio de Educacion y Ciencia of Spain [AGL 2007-60077]
- Comunidad de Madrid (Spain) [S-0505/AGR/000265]
A real-time polymerase chain reaction assay using primers targeting the porcine-specific mitochondrial 125 rRNA gene and universal eukaryotic primers amplifying a conserved fragment of the nuclear 18S rRNA gene has been developed for the detection and quantification of porcine DNA in food and feedstuffs. The 18S rRNA primers were used as endogenous control for the total content of PCR-amplifiable DNA in the sample. The assay was tested on DNA extracted from raw and heat-treated binary mixtures of porcine tissues in a plant matrix, and on DNA extracted from reference feedstuff samples. Analysis of experimental mixtures demonstrated the suitability of the assay for the detection and quantification of porcine DNA in mixtures containing as little as 0.1%. (c) 2009 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据