期刊
MEAT SCIENCE
卷 81, 期 1, 页码 65-70出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.meatsci.2008.06.019
关键词
Salmonella typhimurium; Acid Tolerance Response; Acid adaptation; Food safety
资金
- Junta de Castilla y Leon [LE045A06]
- Spanish Ministry of Education
- University of Leon
Salmonella typhimurium inactivation at pH 3.0 in Brain Heart Infusion (BHI) and Meat Extract (ME) was Studied using stationary-phase cells grown in non-acidified BHI (pH 7.4) and ME (pH 6.6) and acidified BHI and ME at pH Values of 6.4. 5.4 and 4.5 with acetic, ascorbic, citric, lactic malic and hydrochloric, acids. Cells grown in buffered BHI (pH 7.0) were used as non-acid adapted control cells. Acid adapted S. typhimurium cells obtained in both media (BHI and ME) were more resistant to extremely acidic conditions when ME was used as challenge medium, although the ability of S. typhimurium to survive extreme pH conditions also depended on growth medium and type of acidulant used. Acid adapted cells grown in BHI developed a higher Acid Tolerance Response (ATR) than those grown in ME. When cells were grown in acidified BHI, no bacterial inactivation was observed after three hours of acid challenge in ME. Furthermore, when cells were grown in acidified ME at pH values of 6.4 and 5.4, D-values obtained using ME as challenge medium were, respectively, 6-9 and 10-15 fold higher than those found when BHI was used as challenge medium, In all cases, the order of acids in inducing the ATR was citric > acetic > lactic > malic >= hydrochloric > ascorbic. These findings represent a concern for food safety as the increase in the acid resistance of acid adapted cells Could allow for S. typhimurium Survival in the strong acidic environment of the gastrointestinal tract. (C) 2008 Elsevier Ltd. All rights reserved.
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