期刊
MATRIX BIOLOGY
卷 37, 期 -, 页码 49-59出版社
ELSEVIER
DOI: 10.1016/j.matbio.2014.05.007
关键词
Thrombospondin-1; CD47; Extracellular vesicles; Intercellular communication
资金
- Intramural Research Program of the Center for Cancer Research, National Cancer Institute [ZIA SC 009172]
- Intramural Research Program of the National Institute of Allergy and Infectious Diseases
- Intramural Research Program of the National Human Genome Research Institute
Intercellular communication is critical for integrating complex signals in multicellular eukaryotes. Vascular endothelial cells and T lymphocytes closely interact during the recirculation and trans-endothelial migration of T cells. In addition to direct cell-cell contact, we show that T cell derived extracellular vesicles can interact with endothelial cells and modulate their cellular functions. Thrombospondin-1 and its receptor CD47 are expressed on exosomes/ectosomes derived from T cells, and these extracellular vesicles are internalized and modulate signaling in both T cells and endothelial cells. Extracellular vesicles released from cells expressing or lacking CD47 differentially regulate activation of T cells induced by engaging the T cell receptor. Similarly, T cell-derived extracellular vesicles modulate endothelial cell responses to vascular endothelial growth factor and tube formation in a CD47-dependent manner. Uptake of T cell derived extracellular vesicles by recipient endothelial cells globally alters gene expression in a CD47-dependent manner. CD47 also regulates the mRNA content of extracellular vesicles in a manner consistent with some of the resulting alterations in target endothelial cell gene expression. Therefore, the thrombospondin-1 receptor CD47 directly or indirectly regulates intercellular communication mediated by the transfer of extracellular vesicles between vascular cells. (C) 2014 The Authors. Published by Elsevier B.V.
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