期刊
MATRIX BIOLOGY
卷 40, 期 -, 页码 46-53出版社
ELSEVIER
DOI: 10.1016/j.matbio.2014.08.017
关键词
Tenascin; Wnt; beta-Catenin; Whisker; Vibrissa; Stem cell niche
资金
- Swiss National Science Foundation
Whisker follicles have multiple stem cell niches, including epidermal stem cells in the bulge as well as neural crest-derived stem cells and mast cell progenitors in the trabecular region. The neural crest-derived stem cells are a pool of melanocyte precursors. Previously, we found that the extracellular matrix glycoproteins tenascin-C and tenascin-W are expressed near CD34-positive cells in the trabecular stem cell niche of mouse whisker follicles. Here, we analyzed whiskers from tenascin-C knockout mice and found intrafollicular adipocytes and supernumerary mast cells. As Wnt/beta-catenin signaling promotes melanogenesis and suppresses the differentiation of adipocytes and mast cells, we analyzed beta-catenin subcellular localization in the trabecular niche. We found cytoplasmic and nuclear beta-catenin in wild-type mice reflecting active Wnt/beta-catenin signaling, whereas beta-catenin in tenascin-C knockout mice was mostly cell membrane-associated and thus transcriptionally inactive. Furthermore, cells expressing the Wnt/beta-catenin target gene cyclin D1 were enriched in the CD34-positive niches of wild-type compared to tenascin-C knockout mice. We then tested the effects of tenascins on this signaling pathway. We found that tenascin-C and tenascin-W can be co-precipitated with Wnt3a. In vitro, substrate bound tenascins promoted beta-catenin-mediated transcription in the presence of Wnt3a, presumably due to the sequestration and concentration of Wnt3a near the cell surface. We conclude that the presence of tenascin-C in whiskers assures active Wnt/beta-catenin signaling in the niche thereby maintaining the stem cell pool and suppressing aberrant differentiation, while in the knockout mice with reduced Wnt/beta-catenin signaling, stem cells from the trabecular niche can differentiate into ectopic adipocytes and mast cells. (C) 2014 Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/10/).
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