Accelerated wound healing and its promoting effects of biomimetic collagen matrices with siderophore loaded gelatin microspheres in tissue engineering

The prolonged inflammation and elevation of Matrix Metalloproteniases (MMPs) at the wound site causes significant degradation of Extracellular matrix (ECM) which cause delays the process of wound healing. Hence the development of therapeutic dressing matrices to control and to positively regulate MMPs balance was considered important in achieving faster healing. The design of biomaterial matrices of collagen scaffold has the challenge to mimic the function of ECM and emulate to the attraction of fibroblast migration at wound site. Herein, we report the fabricated Collagen (COL) matrices impregnated with Siderophore loaded Gelatin Microspheres (SGM) as a delivery system to control both infection and protease levels in the wound site for accelerated healing. The fabricated collagen scaffold impregnated with siderophore loaded gelatin microspheres (COL-SGM) was characterized physiochemically using Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM) and swelling behaviour. The COL-SGM scaffold possesses good swelling ability and also exhibited better morphology for the cell adhesion and proliferation. The in vitro biocompatibility and in vitro fluorescence activity of the developed scaffold revealed to possess good cell proliferation and migration against NTH 3T3 fibroblast and Human keratinocytes (HaCaT) cell lines. Furthermore, the in vivo evaluation offered the advantage of neutralizing the excessive proteases and delivered the siderophore in controlled fashion depending on the level of wound exudates with modulated MMPs. Moreover, the COL-SGM scaffold exhibited with increase in the collagen synthesis and faster reepitheliazation of wounds. Thus the developed COL-SGM scaffold achieved improvements in biocompatibility and act as a potent MMP inhibitor to improve wound healing efficiency in tissue engineering application.