期刊
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
卷 42, 期 -, 页码 264-272出版社
ELSEVIER
DOI: 10.1016/j.msec.2014.05.034
关键词
Cuttlefish bone; Poly(epsilon-caprolactone); Hydroxyapatite scaffold; Tissue engineering; Cell differentiation
资金
- Ministry of Science, Education and Sports of the Republic of Croatia [125-1252970-3005]
- Spanish Ministry project [DPI2010-20399-C04-03]
- L'Oreal ADRIA-UNESCO national fellowship program for Women in Science
In the present study, we examined the potential of using highly porous poly(epsilon-caprolactone) (PCL)-coated hydroxyapatite (HAp) scaffold derived from cuttlefish bone for bone tissue engineering applications. The cell culture studies were performed in vitro with preosteoblastic MC3T3-E1 cells in static culture conditions. Comparisons were made with uncoated HAp scaffold. The attachment and spreading of preosteoblasts on scaffolds were observed by Live/Dead staining Kit. The cells grown on the HAp/PCL composite scaffold exhibited greater spreading than cells grown on the HAp scaffold. DNA quantification and scanning electron microscopy (SEM) confirmed a good proliferation of cells on the scaffolds. DNA content on the HAp/PCL scaffold was significantly higher compared to porous HAp scaffolds. The amount of collagen synthesis was determined using a hydroxyproline assay. The osteoblastic differentiation of the cells was evaluated by determining alkaline phosphatase (ALP) activity and collagen type I secretion. Furthermore, cell spreading and cell proliferation within scaffolds were observed using a fluorescence microscope. (C) 2014 Elsevier B.V. All rights reserved.
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