期刊
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
卷 44, 期 -, 页码 411-416出版社
ELSEVIER
DOI: 10.1016/j.msec.2014.08.010
关键词
Zinc; Human-like collagen; S-acetylmercaptosuccinic anhydride
资金
- National Natural Science Foundation of China [21276210, 21206135, 21376190]
- National High Technology Research and Development Program of China (863 Program) [2012AA021203, 2014AA022108]
- Shaanxi Provincial Scientific Technology Research and Development Program [2007 K06-03]
To improve zinc binding ability to human-like collagen (HLC) and stability of metal complex, HLC was thiolated by mercaptosuccinylation reaction with S-acetylmercaptosuccinic anhydride (S-AMSA) at pH 8.0. One mole of thiolated HLC-Zn (SHLC-Zn) complex possessed 24.3 mol zinc ions when pH was 8.0 and zinc concentration was 15 mM. The physicochemical properties and biocompatibility of thiolated HLC-Zn (SHLC-Zn) complex were investigated by UV-vis, CD, electrophoresis analysis, differential scanning calorimetry (DSC) and cell viability assay, respectively. The results showed that SHLC-Zn complex(1) exhibited higher zinc ions than that of native HLC and still maintained the secondary structure of HLC though interaction occurred between SHLC and zinc ions, (2) increased the apparent molecular weight when compared with native HLC, (3) exhibited greater thermal stability than native HLC, and (4) presented toxicity free for BHK cells. This study suggests that the SHLC-Zn complex is a potential nutrition as well as zinc supplement in the medical application. (C) 2014 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据