期刊
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
卷 33, 期 7, 页码 3851-3857出版社
ELSEVIER
DOI: 10.1016/j.msec.2013.05.022
关键词
Graphene; Pyrenebutyric acid; DNA biosensor; Methylene blue
资金
- National Natural Science Foundation of China [21275127]
- Key Research Foundation of Fujian Education Department [JA11166]
- Natural Science Foundation of Fujian Province [2011J01059]
- Program for New Century Excellent Talents in Fujian Province University [JA12204]
- Science and Technology project of SIQSAQ [2011QK217]
A stable and uniform organic-inorganic nanocomposite that consists of graphene (GR) and pyrenebutyric acid (PBA) was obtained by ultrasonication, which was characterized by scanning electron microscopy (SEM) and UV-vis absorption spectra. The dispersion was dropped onto a gold electrode surface to obtain GR-PBA modified electrode (GR-PBA/Au). Electrochemical behaviors of the modified electrode were characterized by cyclic voltammetry and electrochemical impedance spectroscopy using [Fe(CN)(6)](3-/4-) as the electroactive probe. A novel DNA biosensor was constructed based on the covalent coupling of amino modified oligonucleotides with the carboxylic group on PBA. By using methylene blue (MB) as a redox-active hybridization indicator, the biosensor was applied to electrochemically detect the complementary sequence, and the results suggested that the peak currents of MB showed a good linear relationship with the logarithm values of target DNA concentrations in the range from 1.0 x 10(-15) to 5.0 x 10(-12) M with a detection limit of 3.8 x 10(-16) M. The selectivity experiment also showed that the biosensor can well distinguish the target DNA from the non-complementary sequences. (C) 2013 Elsevier B.V. All rights reserved.
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