期刊
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
卷 33, 期 7, 页码 3875-3880出版社
ELSEVIER
DOI: 10.1016/j.msec.2013.05.024
关键词
RF plasma; Ethylenediamine; Plasma polymerization; Implant coating; Aging effects; Osteoblast adhesion
资金
- Federal Ministry of Education and Research (Germany) within the research association Campus PlasmaMed, PlasmaImp [13 N11182, 13 N9775, 13 N9779, 13 N11188]
Thin plasma polymer films from ethylenediamine were deposited on planar substrates placed on the powered electrode of a low pressure capacitively coupled 13.56 MHz discharge. The chemical composition of the plasma polymer films was analyzed by Fourier Transform Infrared Reflection Absorption Spectroscopy (FT-IRRAS) as well as by X-ray photoelectron spectroscopy (XPS) after derivatization of the primary amino groups. The PPEDA films undergo an alteration during the storage in ambient air, particularly, due to reactions with oxygen. The molecular changes in PPEDA films were studied over a long-time period of 360 days. Simultaneously, the adhesion of human osteoblast-like cells MG-63 (ATCC) was investigated on PPEDA coated corundum blasted titanium alloy (Ti-6Al-4V), which is applied as implant material in orthopedic surgery. The cell adhesion was determined by flow cytometry and the cell shape was analyzed by scanning electron microscopy. Compared to uncoated reference samples a significantly enhanced cell adhesion and proliferation were measured for PPEDA coated samples, which have been maintained after long-time storage in ambient air and additional sterilization by gamma-irradiation. (C) 2013 Elsevier B.V. All rights reserved.
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