期刊
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
卷 32, 期 8, 页码 2452-2458出版社
ELSEVIER
DOI: 10.1016/j.msec.2012.07.021
关键词
Allogeneic cell transplantation; Mesenchymal stem cells; Amniotic mesenchymal cells; Bone regenerative therapy; Calcium phosphate scaffold
资金
- Japan Society for the Promotion of Science KAKENHI [22791966]
- Grants-in-Aid for Scientific Research [22590498, 22791966] Funding Source: KAKEN
Autogenous mesenchymal stem cells (MSCs) have therapeutic applications in bone regenerative therapy due to their pluripotency. However, the ability of MSCs to proliferate and differentiate varies between donors. Furthermore, alternative sources of MSCs are required for patients with contraindications to autogenous cell therapy. The aim of this study was to evaluate the potential of mesenchymal cells from the human amniotic membrane (HAM) as a source of cells for allogeneic transplantation in bone regenerative therapy. Cells that retained a proliferative capacity of more than 50 population doubling level were distinguished from other HAM cells as HAM alpha cells and induced to osteogenic status-their in vivo osteogenesis was subsequently investigated in rats. It was found that HAM alpha cells were spindle shaped and were positive for MSC markers and negative for hematopoietic stem cell markers. Alkaline phosphatase activity and calcium deposition increased with osteogenic status of HAM alpha cells. The expression of osteocalcin mRNA was increased in HAM alpha cells cultured on calcium phosphate scaffolds. Moreover, xenografted HAM alpha cells remained viable and produced extracellular matrix for several weeks. Thus, this study suggests that human amniotic mesenchymal cells possess osteogenic differentiation potential and could be applied to allogeneic transplantation in bone regenerative therapy. (C) 2012 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据