期刊
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
卷 31, 期 5, 页码 1115-1120出版社
ELSEVIER
DOI: 10.1016/j.msec.2011.04.008
关键词
Glucose oxidase; Biosensor; GOX; Nanodiamond; Amperometric current; Immobilization; Electrochemistry
资金
- NSF [070734232]
- Universidad del Norte
The importance of nanodiamond in biological and technological applications has been recognized recently, and applied in drug delivery, biochip, sensors and biosensors. Under this investigation, nanodiamond (ND) and nitrogen doped nanodiamond (NND) were deposited on n-type silicon films, and later functionalized with enzyme Glucose oxidase (GOX). The GOX functionalized doped and undoped ND films were characterized using combination of several techniques; i.e. FUR spectroscopy, Raman spectroscopy, atomic force microscopy (AFM) and electrochemical techniques. ND/GOX and NND/GOX thin films on n-type silicon have been found to provide sensitive glucose sensor. GOX has been chosen as a model enzyme system to functionalize with ND at molecular level to understand the glucose biosensor. (C) 2011 Elsevier B.V. All rights reserved.
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