期刊
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
卷 30, 期 8, 页码 1260-1265出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.msec.2010.07.006
关键词
Membrane-destabilizing peptides; Cellular uptake; Endosomal escape; Gene therapy; Non-viral vector
资金
- National Natural Science Foundation of China [30970733, 30901175]
- National Basic Research Program of China [2007CB935603, 2010CB732402]
- Program for New Century Excellent Talents in Fujian Province University.
One of the crucial steps in gene delivery with non-viral vectors is the escape of DNA complexes from the endosome. In order to improve gene transfection efficiency, we designed a novel gene delivery vector gelatin-siloxane nanoparticles (GS NPs) conjugated with two different membrane-destabilizing peptides, octaarginine (R8) and a subunit of influenza virus haemagglutinin HA2. Both R8-GS NPs and HA2-GS NPs had high positive surface charges. They could condense and protect DNA against serum/DNase degradation. Results from flow cytometry and confocal laser scanning microscope respectively indicated that R8-GS NPs had higher uptake efficiency than HA2-GS NPs, whereas HA2-GS had higher endosome escaping efficiency. Furthermore, in vitro transfection displayed a higher gene expression level with HA2-modified GS NPs, which suggested that endosome escaping is the crucial step for nanoparticle mediated gene therapy. (C) 2010 Elsevier B.V. All rights reserved.
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