期刊
MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS
卷 29, 期 1, 页码 50-54出版社
ELSEVIER
DOI: 10.1016/j.msec.2008.05.005
关键词
Carbon nanotubes; Chitosan; Cold nanoparticles; Glucose oxidase; Biosensor
资金
- Specialized Research Fund for the Doctoral Program of Higher Education [SRFDP20060532006]
A new amperometric glucose biosensor was constructed, based on the immobilization of glucose oxidase (GOx) with cross-linking in the matrix of chitosan on a glassy carbon electrode, which was modified by layer-by-layer assembled carbon nanotube (CNT)/chitosan (CHIT)/gold nanoparticles (GNp) multilayer films. With the increasing of CNT/CHIT/GNp layers, the response current to H2O2 was changed regularly and the response current reached a maximum value when the number of CNT/CHIT/GNp layers was 8. The assembling process of multilayer films was simple to operate. With GOx as an enzyme model, a new glucose biosensor was fabricated. The excellent electocatalytic activity and special structure of the enzyme electrode resulted in good characteristics. The linear range was 6 x 10(-6) similar to 5 x 10(-3) M, with a detection limit of 3 x 10(-6) M estimated at a signal-to-noise ratio of 3, fast response time (less than 6 s). Moreover, it exhibited good reproducibility and stability. (C) 2008 Elsevier B.V. All rights reserved.
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