Monosize magnetic hydrophobic beads for lysozyme purification under magnetic field

Monosize and magnetic poly(glycidyl methacrylate-N-methacryloyl-(L)-tryptophan) [mPGMATrp] beads (1.6 mu m in diameter) were used for hydrophobic affinity capture of lysozyme from chicken egg-white. N-methacryloyl-(L)-tryptophan (MATrp), which gives hydrophobicity to the resulting polymer, was synthesized by reacting methacryloyl chloride and L-tryptophan methyl ester then characterized by Nuclear Magnetic Resonance (NMR). mPGMATrp beads were produced by dispersion polymerization in the presence of magnetite nano-powder. mPGMATrp beads were characterized by means of swelling studies, elemental analysis, Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscope (SEM). Lysozyme adsorption experiments were performed under different experimental conditions (i.e., lysozyme concentration. temperature. and ionic strength) in magnetically stabilized fluidized bed system, (MSFB). Maximum adsorption capacity was 263.9 mg/g. It was observed that mPGMATrp beads call be used without significant loss in lysozyme adsorption capacity after 25 adsorption-elution Cycle. (C) 2009 Elsevier B.V. All rights reserved.