期刊
MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS
卷 28, 期 8, 页码 1372-1375出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.msec.2008.03.001
关键词
Nanocomposite material; Glucose biosensor; Laponite; Chitosan; Glucose
资金
- National Natural Science Foundation of China [20505014]
- scientific research foundation for the returned overseas Chinese scholars, state education ministry
Nanocomposite matrix based on chitosan/laponite was Successfully utilized to construct a new type of amperometric glucose biosensor. This hybrid material combined the merits of organic biopolymer, chitosan, and synthesized inorganic clay, laponite. Glucose oxidase (GOD) immobilized in the material maintained its activity well as the usage of glutaraldehyde was avoided. The composite films were characterized by Fourier transform infrared (FT-IR). The parameters affecting the fabrication and experimental conditions of biosensors were optimized. The sensitivity of the proposed biosensor (33.9 mA M-1 cm(-2)) permitted the determination of glucose in the concentration range of 1 x 10(-6) -5 x 10(-5) M with a detection limit of 0.3 mu M based on S/N=3. The apparent Michaelis-Menten constant (K-M(app)) for the sensor was found to be 15.8 mM. (c) 2008 Elsevier B.V. All rights reserved.
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