Using a synthetic body-fluid (SBF) solution of 27 mM HCO3- to make bone substitutes more osteointegrative

A Tris-HCl-buffered synthetic body fluid (SBF) solution, mimicking the human blood plasma, with the following ion concentrations of 27 mM HCO3- 2.5 mM Ca2+, 1.0 mM HPO42-, 142 mM Na+, 125 mM Cl-, 5 mM K+, 1.5 mM Mg2+ and 0.5 mM SO42- was used as an aqueous medium to process a number of bone substitute materials under the so-called biomimetic conditions of 37 degrees C and pH 7.4. This solution was named as Tris-SBF-27 mM. Firstly, collagen sponges were soaked in Tris-SBF-27 mM solution at 37 degrees C and were found to be fully covered with nanoporous apatitic calcium phosphate (Ap-CaP). The composites of collagen-Ap-CaP biomaterials are expected to be used in orthopedic and dental surgery. Secondly, Ap-CaP short whiskers or microrods with a novel nanotexture and surface areas higher than 45 m(2)/g were synthesized in Tris-SBF-27 mM solution. Thirdly, calcium sulfate cements doped with CaHPO4 (monetite), were shown to have apatite-inducing ability upon ageing in Tris-SBF-27 mM. CaHPO4 addition in calcium sulfate was found to improve its mechanical strength, measured after cement setting reaction. Pure calcium sulfate cement pellets were not stable in Tris-SBF-27 mM solutions and crumbled into a powder. All the samples were characterized by SEM, XRD, FTIR, surface area and mechanical strength measurements. (C) 2007 Elsevier B.V. All rights reserved.