期刊
RESEARCH IN VETERINARY SCIENCE
卷 99, 期 -, 页码 149-156出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.rvsc.2015.01.008
关键词
Probiotic; Bacillus amyloliquefaciens; Soybean; Cellulolytic; Animal feed
资金
- UGC-RGNF New Delhi [F1 - 17.1/2011 - 12/RGNF-SC-CHH-1645]
- DBT [BT/219/NE/TBP/2011]
- Strengthening of teaching, training and research in Universities and Colleges of North-East India
- Ministry of Science and Technology, New Delhi
- UGC-SAP
- DST-FIST
- University Grants Commission, India
A microorganism showing probiotic attributes and hydrolyzing carboxymethylcellulose was isolated from traditional fermented soybean (Churpi) and identified as Bacillus amyloliquefaciens by analysis of 16S rRNA gene sequence and named as B. amyloliquefaciens AMS1. The potentiality of this isolate as probiotic was investigated in vitro and it showed gastrointestinal transit tolerance, cell surface hydrophobicity, cell aggregation and antimicrobial activity. The isolate was found to be non-hemolytic which further strengthens its candidature as a potential probiotic. The maize straw digestion was confirmed by scanning electron microscopy studies. The isolate was able to degrade filter paper within 96 hours of incubation. This study explores the possibility of combining the cellulase degrading ability of a microbe with its probiotic attributes to enhance gut health of animal and digestibility of the feed. (C) 2015 Elsevier Ltd. All rights reserved.
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