期刊
MATERIALS CHEMISTRY AND PHYSICS
卷 126, 期 3, 页码 470-475出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.matchemphys.2010.12.060
关键词
Nanostructures; Fourier transform infrared spectroscopy; Electron microscopy
资金
- State Key Basic Research (973) Program [2007CB936100]
- National Hi-Tech Research Development (863) Program [2009AA03Z311]
- Foundation of Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin University of Science and Technology [Wsw-01]
Two-dimensional layered materials exhibit desired functionalities when being used as gene delivery materials. In this study, a novel gene delivering vector, lamellar hydroxyapatite (HAp)/DNA nanohybrid was prepared. The structure of HAp/DNA nanohybrid was investigated by X-ray diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Fourier transform infrared (FT-IR) spectroscopy analysis revealed that ion-exchange occurred during the process. Gel electrophoresis analysis confirmed that the lamellar HAp could protect DNA from degradation of DNase I and the protected DNA could be recovered readily under acid conditions. Furthermore, the integrity of released DNA was confirmed by UV-vis spectra. (C) 2010 Elsevier B.V. All rights reserved.
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