期刊
MASS SPECTROMETRY REVIEWS
卷 33, 期 3, 页码 157-182出版社
WILEY
DOI: 10.1002/mas.21389
关键词
HSAB theory; electrophilic lipoxidation products; HNE; aldehyde-reactive probe; collision induced dissociation; electron capture dissociation; electron transfer dissociation; ion mobility mass spectrometry
类别
资金
- National Institutes of Health [R01AG025372, S10RR025628, P30ES000210, R01RR026275]
- National Science Foundation [0924027]
- OSU RERF
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [0924027] Funding Source: National Science Foundation
The post-translational modification of proteins by electrophilic oxylipids is emerging as an important mechanism that contributes to the complexity of proteomes. Enzymatic and non-enzymatic oxidation of biological lipids results in the formation of chemically diverse electrophilic carbonyl compounds, such as 2-alkenals and 4-hydroxy alkenals, epoxides, and eicosanoids with reactive cyclopentenone structures. These lipoxidation products are capable of modifying proteins. Originally considered solely as markers of oxidative insult, more recently the modifications of proteins by lipid peroxidation products are being recognized as a new mechanism of cell signaling with relevance to redox homeostasis, adaptive response and inflammatory resolution. The growing interest in protein modifications by reactive oxylipid species necessitates the availability of methods that are capable of detecting, identifying and characterizing these protein adducts in biological samples with high complexity. However, the efficient analysis of these chemically diverse protein adducts presents a considerable analytical challenge. We first provide an introduction into the chemistry and biological relevance of protein adductions by electrophilic lipoxidation products. We then provide an overview of tandem mass spectrometry approaches that have been developed in recent years for the interrogation of protein modifications by electrophilic oxylipid species. (c) 2013 Wiley Periodicals, Inc. Mass Spec Rev 33: 157-182, 2014.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据