期刊
MASS SPECTROMETRY REVIEWS
卷 28, 期 2, 页码 273-361出版社
WILEY
DOI: 10.1002/mas.20192
关键词
MALDI; carbohydrates; glycoproteins; glycolipids; fragmentation; biopharmaceuticals; glycosyltransferases; glycosidases; time-of-flight
类别
This review is the third update of the original review, published in 1999, on the application of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings the topic to the end of 2004. Both fundamental studies and applications tire covered. The main topics include methodological developments, matrices, fragmentation of carbohydrates and applications to large polymeric carbohydrates from plants, glycans from glycoproteins and those from various glycolipids. Other topics include the use of MALDI MS to study enzymes related to carbohydrate biosynthesis and degradation, its use in industrial Processes, particularly biopharmaceuticals and its use to monitor products of chemical synthesis where glycodendrimers and carbohydrate-protein complexes are highlighted. (C) 2008 Wiley Periodicals, Inc., Mass Spec Rev 28:273-361, 2009
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