期刊
MARINE POLLUTION BULLETIN
卷 85, 期 2, 页码 816-823出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.marpolbul.2014.04.043
关键词
Traditional Chinese Medicine; Lactococcus garvieae; Grey mullet; Bactericidal activity; Aquaculture
Lactococcosis is prevalent on grey mullet (Mugil cephalus) in Hong Kong aquaculture resulting in serious economic loss. A compound formulation of Traditional Chinese Medicines (TCM) (modified Huanglian Jiedu decoction (HLJDD)) comprising Rhizoma coptidis, Radix scutellaria, Cortex phellodendri, Fructus gardeniae, Fructus forsythiae and Flos lonicerae japonicae (in a ratio of 3:2:2:3:3:5) were applied as feed supplements to deal with the disease. The Nitroblue tetrazolium activity in blood, bactericidal activity and total immunoglobulin in plasma were significantly enhanced after feeding 1% of this TCM for 28 days. The disease resistances to Lactococcus garvieae in 1% and 2% TCM feeding groups were significantly enhanced. In the in vitro study, the modified HLJDD also activated the plasma bactericidal activities (p < 0.01). Based on this study, 1% modified HLJDD feeding for 28 days may be an optimal dose to prevent L garvieae infection and could be used in aquaculture industries. (C) 2014 Elsevier Ltd. All rights reserved.
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