期刊
MARINE POLLUTION BULLETIN
卷 63, 期 5-12, 页码 277-286出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.marpolbul.2011.04.023
关键词
Polychaete; Neanthes succinea; Copper and zinc superoxide dismutase; Manganese superoxide dismutase; Oxidative stress; Copper
资金
- National Research Foundation [2010-0015903]
To evaluate the superoxide dismutase (SOD) genes as a potential indicator of environmental stress, we cloned and sequenced the full-length cDNA of a Cu/Zn-SOD and Mn-SOD genes from the marine polychaete, Neanthes succinea. Ns-Cu/Zn- and Mn-SOD expression was measured using quantitative real-time RT-PCR in response to the oxidative stress induced by copper in the form of CuCl2. Ns-Cu/Zn-SOD expression increased in a dose dependent manner but the increase of Mn-SOD expression was evident only at a high concentration of copper. Particularly, Ns-Mn-SOD dramatically increased in the 72 mu g/L CuCl2-exposed groups, indicating that both Ns-SOD genes were induced by copper exposure. These results suggest that Ns-Cu/Zn- and Ns-Mn-SOD would play important roles in the detoxification of cellular oxidative stresses in the polychaete, N. succinea. Furthermore, as part of field work, the marine polychaete, N. succinea Cu/Zn-SOD mRNA collected from the polluted area was accordingly upregulated in expression but not for NS-Mn-SOD. (C) 2011 Elsevier Ltd. All rights reserved.
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