期刊
MARINE GENOMICS
卷 18, 期 -, 页码 163-171出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.margen.2014.06.006
关键词
Antarctic notothenioid; Cold-specialization; 4 degrees C warming; RNA-seq; HSP down regulation; Slowing cell cycle
资金
- United States National Science Foundation Polar Programs [ANT-0231006, ANT-1142158]
- Natural Science Foundation of China [30910103906]
- Directorate For Geosciences
- Office of Polar Programs (OPP) [1142158] Funding Source: National Science Foundation
Through evolution in the isolated, freezing (-1.9 degrees C) Southern Ocean, Antarctic notothenioid fish have become cold-adapted as well as cold-specialized. Notothenioid cold specialization is most evident in their limited tolerance to heat challenge, and an apparent loss of the near universal inducible heat shock (HSP70) response. Beyond these it remains unclear how broadly cold specialization pervades the underlying tissue-wide cellular responses. We report the first analysis of massively parallel RNA sequencing (RNA-seq) to identify gene expression changes in the liver in response to elevated body temperature of a high-latitude Antarctic nototheniid, the highly coldadapted and cold-specialized cryopelagic bald notothen, Pagothenia borchgrevinki. From a large (14,873) mapped set of qualified, annotated liver transcripts, we identified hundreds of significantly differentially expressed genes following two and four days of 4 degrees C exposure, suggesting substantial transcriptional reorganization in the liver when body temperature was raised 5 degrees C above native water temperature. Most notably, and in sharp contrast to heat stressed non-polar fish species, was a widespread down-regulation of nearly all classes of molecular chaperones including HSP70, as well as polyubiquitins that are associated with proteosomal degradation of damaged proteins. In parallel, genes involved in the cell cycle were down-regulated by day two of 4 degrees C exposure, signifying slowing cellular proliferation; by day four, genes associated with transcriptional and translational machineries were down-regulated, signifying general slowing of protein biosynthesis. The log2 fold differential transcriptional changes are generally of small magnitudes but significant, and in total portray a broad down turn of cellular activities in response to four days of elevated body temperature in the cold-specialized bald notothen. (c) 2014 Elsevier B.V. All rights reserved.
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