期刊
MARINE DRUGS
卷 12, 期 4, 页码 2205-2227出版社
MDPI
DOI: 10.3390/md12042205
关键词
Delesseria sanguinea; sulfated polysaccharides; quality control; fluorescence sensor; (red) algae; biopolymer; assay development; Polymer-H; sulfated glycan; fondaparinux
资金
- European Fishery Fund (EFF) of the European Union
- Ministry of Agriculture, Environment and Consumer Protection Mecklenburg-Vorpommern
Sulfated polysaccharides (SP) from algae are of great interest due to their manifold biological activities. Obstacles to commercial (especially medical) application include considerable variability and complex chemical composition making the analysis and the quality control challenging. The aim of this study was to evaluate a simple microplate assay for screening the quality of SP. It is based on the fluorescence intensity (FI) increase of the sensor molecule Polymer-H by SP and was originally developed for direct quantification of SP. Exemplarily, 65 SP batches isolated from the red alga Delesseria sanguinea (D.s.-SP) and several other algae polysaccharides were investigated. Their FI increase in the Polymer-H assay was compared with other analytical parameters. By testing just one concentration of a D.s.-SP sample, quality deviations from the reference D.s.-SP and thus both batch-to-batch variability and stability can be detected. Further, structurally distinct SP showed to differ in their concentration-dependent FI profiles. By using corresponding reference compounds, the Polymer-H assay is therefore applicable as identification assay with high negative predictability. In conclusion, the Polymer-H assay showed to represent not only a simple method for quantification, but also for characterization identification and differentiation of SP of marine origin.
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