期刊
MARINE DRUGS
卷 11, 期 11, 页码 4390-4406出版社
MDPI
DOI: 10.3390/md11114390
关键词
pigments; microalgae; Cyanophora paradoxa; melanoma; cancer; apoptosis; zeaxanthin; cryptoxanthin
资金
- European FEDER funds [34755-2011]
- CPER Contrats de Projet Etat-Region: Poitou-Charentes funds (Project Extraction of anticancer pigments from marine microalgae)
- French cancer league (Comite 17 de la Ligue Nationale contre le Cancer)
The glaucophyte Cyanophora paradoxa (Cp) was chemically investigated to identify pigments efficiently inhibiting malignant melanoma, mammary carcinoma and lung adenocarcinoma cells growth. Cp water and ethanol extracts significantly inhibited the growth of the three cancer cell lines in vitro, at 100 mu g.mL(-1). Flash chromatography of the Cp ethanol extract, devoid of c-phycocyanin and allophycocyanin, enabled the collection of eight fractions, four of which strongly inhibited cancer cells growth at 100 mu g.mL(-1). Particularly, two fractions inhibited more than 90% of the melanoma cells growth, one inducing apoptosis in the three cancer cells lines. The detailed analysis of Cp pigment composition resulted in the discrimination of 17 molecules, ten of which were unequivocally identified by high resolution mass spectrometry. Pheophorbide a, beta-cryptoxanthin and zeaxanthin were the three main pigments or derivatives responsible for the strong cytotoxicity of Cp fractions in cancer cells. These data point to Cyanophora paradoxa as a new microalgal source to purify potent anticancer pigments, and demonstrate for the first time the strong antiproliferative activity of zeaxanthin and beta-cryptoxanthin in melanoma cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据