期刊
MARINE DRUGS
卷 11, 期 8, 页码 2894-2916出版社
MDPI
DOI: 10.3390/md11082894
关键词
algae; biofuel; bioprocess scale-up; metabolism; systems biology
资金
- US National Science Foundation
- National Basic Research Program of China (973 Project) [2012CB721006]
- Specialized Research Fund for the Doctoral Program of Higher Education of China [20100146120021]
- I-CARES at Washington University
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [0954016] Funding Source: National Science Foundation
Cyanobacteria (blue-green algae) play profound roles in ecology and biogeochemistry. One model cyanobacterial species is the unicellular cyanobacterium Synechocystis sp. PCC 6803. This species is highly amenable to genetic modification. Its genome has been sequenced and many systems biology and molecular biology tools are available to study this bacterium. Recently, researchers have put significant efforts into understanding and engineering this bacterium to produce chemicals and biofuels from sunlight and CO2. To demonstrate our perspective on the application of this cyanobacterium as a photosynthesis-based chassis, we summarize the recent research on Synechocystis 6803 by focusing on five topics: rate-limiting factors for cell cultivation; molecular tools for genetic modifications; high-throughput system biology for genome wide analysis; metabolic modeling for physiological prediction and rational metabolic engineering; and applications in producing diverse chemicals. We also discuss the particular challenges for systems analysis and engineering applications of this microorganism, including precise characterization of versatile cell metabolism, improvement of product rates and titers, bioprocess scale-up, and product recovery. Although much progress has been achieved in the development of Synechocystis 6803 as a phototrophic cell factory, the biotechnology for Compounds from Synechocystis is still significantly lagging behind those for heterotrophic microbes (e. g., Escherichia coli).
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