期刊
MARINE DRUGS
卷 10, 期 10, 页码 2138-2152出版社
MDPI
DOI: 10.3390/md10102138
关键词
ESI-CID-MS/MS; fucoidan; oligosaccharide; Saccharina japonica
资金
- Ocean Public Welfare Scientific Research Project
- Doctorial Fund of Shandong Province [BS2011YY066]
- Science and Technology Project of Shandong Province [2011GHY11529]
- Prospective Joint Research Projects of Jiangsu Province [BY2011189]
- State Oceanic Administration of the People's Republic of China [201005024]
A fucoidan extracted from Saccharina japonica was fractionated by anion exchange chromatography. The most complex fraction F0.5 was degraded by dilute sulphuric acid and then separated by use of an activated carbon column. Fraction Y1 was fractionated by anion exchange and gel filtration chromatography while Fraction Y2 was fractionated by gel filtration chromatography. The fractions were determined by ESI-MS and analyzed by ESI-CID-MS/MS. It was concluded that F0.5 had a backbone of alternating 4-linked GlcA and 2-linked Man with the first Man residue from the nonreducing end accidentally sulfated at C6. In addition, F0.5 had a 3-linked glucuronan, in accordance with a previous report by NMR. Some other structural characteristics included GlcA 1 -> 3 Man 1 -> 4 GlcA, Man 1 -> 3 GlcA 1 -> 4 GlcA, Fuc 1 -> 4 GlcA and Fuc 1 -> 3 Fuc. Finally, it was shown that fucose was sulfated at C2 or C4 while galactose was sulfated at C2, C4 or C6.
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