期刊
MARINE DRUGS
卷 9, 期 1, 页码 109-123出版社
MDPI
DOI: 10.3390/md9010109
关键词
bifunctional alginate lyase; Pseudoalteromonas sp SM0524; alginate
资金
- National Natural Science Foundation of China [31025001, 31000034]
- Hi-Tech Research and Development program of China [2007AA091903]
- COMRA Program [DYXM-115-02-2-6]
- Natural Science Funds of Shandong Province, China [JQ200910, BS2010SW015, ZR2009DZ002]
An alginate lyase-producing bacterial strain, Pseudoalteromonas sp. SM0524, was screened from marine rotten kelp. In an optimized condition, the production of alginate lyase from Pseudoalteromonas sp. SM0524 reached 62.6 U/mL, suggesting that strain SM0524 is a good producer of alginate lyases. The bifunctional alginate lyase aly-SJ02 secreted by strain SM0524 was purified. Aly-SJ02 had an apparent molecular mass of 32 kDa. The optimal temperature and pH of aly-SJ02 toward sodium alginate was 50 degrees C and 8.5, respectively. The half life period of aly-SJ02 was 41 min at 40 degrees C and 20 min at 50 degrees C. Aly-SJ02 was most stable at pH 8.0. N-terminal sequence analysis suggested that aly-SJ02 may be an alginate lyase of polysaccharide lyase family 18. Aly-SJ02 showed activities toward both polyG (alpha-L-guluronic acid) and polyM (beta-D-mannuronic acid), indicating that it is a bifunctional alginate lyase. Aly-SJ02 had lower K-m toward polyG than toward polyM and sodium alginate. Thin layer chromatography and ESI-MS analyses showed that aly-SJ02 mainly released dimers and trimers from polyM and alginate, and trimers and tetramers from polyG, which suggests that aly-SJ02 may be a good tool to produce dimers and trimers from alginate.
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