期刊
MARINE BIOTECHNOLOGY
卷 13, 期 5, 页码 992-1002出版社
SPRINGER
DOI: 10.1007/s10126-011-9363-x
关键词
Scleractinian corals; Carbonic anhydrase; Symbiosis; Calcification; Intracelullar pH
资金
- Government of the Principality of Monaco
- Scientific Centre of Monaco
Scleractinian corals are of particular interest due to their ability to establish an intracellular mutualistic symbiosis with phototrophic dinoflagellates and to deposit high rates of calcium carbonate in their skeleton. Carbonic anhydrases have been shown to play a crucial role in both processes. In this study, we report the molecular cloning and characterization of a novel alpha-CA in the coral Stylophora pistillata. This enzyme shares homologies with the human isoform CA II and is referred to as STPCA-2. STPCA-2 is 35.2 kDa and possesses all key amino acids for catalytic activity. With a ratio between catalytic and Michaelis constants (k(cat)/K-m) of 8.3.10(7) M-1 s(-1) is considered as highly active. Owing to its intracellular localisation in the oral endoderm and in the aboral tissue, we propose that STPCA-2 is involved in pH regulation and/or inorganic carbon delivery to symbiont and calcification.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据