期刊
MALARIA JOURNAL
卷 11, 期 -, 页码 -出版社
BIOMED CENTRAL LTD
DOI: 10.1186/1475-2875-11-103
关键词
Plasmodium berghei; Negative selection; Transfection; Selectable marker; Malaria
资金
- EviMalar [242095]
- Wellcome Trust [083811/Z/07/Z]
- Wellcome Trust [083811/Z/07/Z] Funding Source: Wellcome Trust
An improved methodology is presented here for transgenic Plasmodium berghei lines that express the negative selectable marker yFCU (a bifunctional protein that combines yeast cytosine deaminase and uridyl phosphoribosyl transferase (UPRT)) and substitutes delivery of selection drug 5-fluorocytosine (5FC) by intraperitoneal injection for administration via the drinking water of the mice. The improved methodology is shown to be as effective, less labour-intensive, reduces animal handling and animal numbers required for successful selection thereby contributing to two of the three Rs of animal experimentation, namely refinement and reduction.
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