期刊
MAGNETIC RESONANCE IN MEDICINE
卷 74, 期 2, 页码 312-319出版社
WILEY
DOI: 10.1002/mrm.25419
关键词
imaging; metabolism; magnetic resonance spectroscopy; isoproterenol
资金
- NIH [5R37-HL034557, 8P41-EB015908]
PurposeThe diseased myocardium lacks metabolic flexibility and responds to stimuli differently compared with healthy hearts. Here, we report the use of hyperpolarized C-13 NMR spectroscopy to detect sudden changes in cardiac metabolism in isolated, perfused rat hearts in response to adrenergic stimulation. MethodsMetabolism of hyperpolarized [1-C-13]pyruvate was investigated in perfused rat hearts. The hearts were stimulated in situ by isoproterenol shortly after the administration of hyperpolarized [1-C-13]pyruvate. The hyperpolarized C-13 NMR results were corroborated with H-1 NMR spectroscopy of tissue extracts. ResultsAddition of isoproterenol to hearts after equilibration of hyperpolarized [1-C-13]pyruvate into the existing lactate pool resulted in a sudden, rapid increase in hyperpolarized [1-C-13]lactate signal within seconds after exposure to drug. The hyperpolarized (HCO3-)-C-13 and hyperpolarized [1-C-13]alanine signals were not affected by the isoproterenol-induced elevated cardiac workload. Separate experiments confirmed that the new hyperpolarized [1-C-13]lactate signal that arises after stimulation by isoproterenol reflects a sudden increase in total tissue lactate derived from glycogen. ConclusionThese results suggest that hyperpolarized pyruvate and C-13 MRS may be useful for detecting abnormal glycogen metabolism in intact tissues. Magn Reson Med 74:312-319, 2015. (c) 2014 Wiley Periodicals, Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据