期刊
MAGNETIC RESONANCE IN MEDICINE
卷 74, 期 5, 页码 1406-1413出版社
WILEY
DOI: 10.1002/mrm.25530
关键词
hyperpolarized molecules; pH mapping; metabolism
资金
- NIH [01HL116342, R01EB010208]
PurposeTo produce hyperpolarized bicarbonate indirectly via chemical reaction from a hyperpolarized precursor and utilize it for the simultaneous regional measurement of metabolism and pH. MethodsAlpha keto carboxylic acids are first hyperpolarized by dissolution dynamic nuclear polarization (DNP). These precursor molecules are rapidly reacted with hydrogen peroxide (H2O2) to decarboxylate the species, resulting in new target molecules. Unreacted H2O2 is removed from the system by reaction with sulfite. Interrogation of the ratio of dissolved carbon dioxide (CO2) to bicarbonate can be used to determine pH. ResultsConversion of hyperpolarized alpha keto acids to bicarbonate and CO2 results in a minimal loss of the spin order. The reaction can be conducted to completion within seconds and preserves the nuclear spin polarization. ConclusionThrough a rapid chemical reaction, we can conserve the nuclear spin order of a DNP precursor to generate multiple hyperpolarized bioprobes otherwise unamenable to polarization. This indirect technique for the production of hyperpolarized agents can be applied to different precursor compounds to generate additional novel probes. Magn Reson Med 74:1406-1413, 2015. (c) 2014 Wiley Periodicals, Inc.
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