NMR spectral assignments of two sterols from a soft coral Sinularia brassica

Two new steroids, named 4 alpha, 22-dimethyl-Cholest-22-en-3 beta-ol (1) and 4 alpha-methyl-Cholest-7,22-dien-3 beta-ol (2), along with two known steroids, were isolated from the soft coral Sinularia brassica. The structures of the new compounds were determined on the basis of extensive spectroscopic data (MS, H-1 and C-13 NMR, H-1-H-1 COSY, C-13-H-1 COSY, HMBC and NOESY) analysis. Copyright (C) 2010 John Wiley & Sons, Ltd.