期刊
MAGNETIC RESONANCE IN CHEMISTRY
卷 46, 期 6, 页码 508-517出版社
JOHN WILEY & SONS LTD
DOI: 10.1002/mrc.2201
关键词
NMR; HSQC; 1-methylimidazole-d(6); dimethylsulfoxide-d(6); Pinus taeda; Populus tremuloides; Hibiscus cannabinus; whole cell wall dissolution; lignin; polysaccharides
资金
- NATIONAL CENTER FOR RESEARCH RESOURCES [S10RR008438, P41RR002301, S10RR002781] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [P41GM066326] Funding Source: NIH RePORTER
- NCRR NIH HHS [P41RR02301, S10 RR002781-01, S10 RR002781, P41 RR002301, S10 RR008438-01, RR08438, S10 RR008438, RR02781] Funding Source: Medline
- NIGMS NIH HHS [P41GM66326, P41 GM066326] Funding Source: Medline
A recently described plant cell wall dissolution system has been modified to use perdeuterated solvents to allow direct in-NMR-tube dissolution and high-resolution solution-state NMR of the whole cell wall without derivatization. Finely ground cell wall material dissolves in a solvent system containing dimethylsulfoxide-d(6) and 1-methylimidazole-d(6) in a ratio of 4:1 (v/v), keeping wood component structures mainly intact in their near-native state. Two-dimensional NMR experiments, using gradient-HSQC (heteronuclear single quantum coherence) 1-bond C-13-H-1 correlation spectroscopy, on nonderivatized cell wall material from a representative gymnosperm Pinus taeda (loblolly pine), an angiosperm Populus tremuloides (quaking aspen), and a herbaceous plant Hibiscus cannabinus (kenaf) demonstrate the efficacy of the system. We describe a method to synthesize 1-methylimidazole-d(6) with a high degree of perdeuteration, thus allowing cell wall dissolution and NMR characterization of nonderivatized plant cell wall structures. Copyright (C) 2008 John Wiley & Sons, Ltd.
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