期刊
MAGNETIC RESONANCE IMAGING
卷 27, 期 5, 页码 648-655出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.mri.2008.10.003
关键词
Cryopreservation; Cartilage; Microscopic MRI; Glycosaminoglycan; dGEMRIC; Biochemical assay; Dimethyl sulfoxide (DMSO); DMMB
资金
- NIH [AR 45172, AR 52353]
- NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES [R01AR045172, R01AR052353] Funding Source: NIH RePORTER
To investigate the damages to the extracellular matrix in articular cartilage due to cryopreservation, the depth-dependent concentration profiles of glycosaminoglycans (GAGs) in 34 cartilage specimens from canine humeral heads were imaged at 13-mu m pixel resolution using the in vitro version of the dGEMRIC protocol in microscopic MRI (mu MRI). In addition, a biochemical assay was used to determine the GAG loss from the tissue to the solution where the tissue was immersed. For specimens that had been frozen at -20 degrees C or -80 degrees C without any cryoprotectant, a significant loss of GAG (as high as 56.5%) was found in cartilage, dependent upon the structural zones of the tissue and the conditions of cryopreservation. The cryoprotective abilities of dimethyl sulfoxide (DMSO) as a function of its concentration in saline and storage temperature were also investigated. A 30% DMSO concentration was sufficient in preventing the reduction of GAG in the tissue at the -20 degrees C storage temperature, but a 50% concentration of DMSO was necessary for the -80 degrees C cryopreservation. These imaging results were verified by the biochemical analysis. (C) 2009 Elsevier Inc. All rights reserved.
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