期刊
REGENERATIVE MEDICINE
卷 10, 期 6, 页码 745-755出版社
FUTURE MEDICINE LTD
DOI: 10.2217/rme.15.45
关键词
collagen; endothelial; extracellular matrix; iPSC; nanofibrillar; scaffolds; smooth muscle; stem cells; tissue engineering; vascular graft
资金
- US NIH [R00HL098688, EB020235-01]
- Department of Veterans Affairs Biomedical Laboratory Research and Development [1I01BX002310]
- Stanford Chemistry Engineering & Medicine for Human Health
- Stanford Cardiovascular Institute
- California Institute of Regenerative Medicine [TR3-05569]
- National Heart, Lung and Blood Institute [1T32HL098049]
- American Heart Association [15POST25560045]
- San Jose State University Consortium for Stem Cell Internships in Laboratory-based Learning
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [T32HL098049, R00HL098688, P50HL083800] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [R21EB020235] Funding Source: NIH RePORTER
- Veterans Affairs [I01BX002310] Funding Source: NIH RePORTER
Background: We developed an aligned bi-layered vascular graft derived from human induced pluripotent stem cells (iPSCs) that recapitulates the cellular composition, orientation, and anti-inflammatory function of blood vessels. Materials & methods: The luminal layer consisted of longitudinal-aligned nanofibrillar collagen containing primary endothelial cells (ECs) or iPSC-derived ECs (iPSC-ECs). The outer layer contained circumferentially oriented nanofibrillar collagen with primary smooth muscle cells (SMCs) or iPSC-derived SMCs(iPSC-SMCs). Results: On the aligned scaffolds, cells organized F-actin assembly within 8 degrees from the direction of nanofibrils. When compared to randomly-oriented scaffolds, EC-seeded aligned scaffolds had significant reduced inflammatory response, based on adhesivity to monocytes. Conclusion: This study highlights the importance of anisotropic scaffolds in directing cell form and function, and has therapeutic significance as physiologically relevant blood vessels.
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