期刊
MACROMOLECULAR RESEARCH
卷 20, 期 11, 页码 1156-1162出版社
POLYMER SOC KOREA
DOI: 10.1007/s13233-012-0164-5
关键词
gene delivery; dendrimer; transfection; amino-acid modification
资金
- National Research Foundation of Korea (NRF)
- Ministry of Education, Science and Technology [20100021647, 2010-0020224]
- Priority Research Centers Program through the National Research Foundation of Korea (NRF)
- National Research Foundation of Korea [2010-0021647, 2010-0020224] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Poly(amidoamine) (PAMAM) dendrimers are synthetic polymers commonly used as carriers in gene and drug delivery. PAMAMs have the ability to transfect DNA, but their transfection efficiency is currently insufficient for clinical use. Here, we demonstrate the synthesis and evaluation of cationic dendrimers consisting of a cystamine core PAMAM generation 3 (cPAM G3) and amino acids. Introduction of histidine (His) and arginine (Arg) residues to cPAM G3 resulted in high transfection efficiency and low cytotoxicity. cPAM G3-His-Arg formed stable polyplexes at a weight ratio of 6:1, and the mean polyplex diameter was 99.03 +/- 1.68 nm. Nano-sized polyplexes increased in diameter up to 132.93 +/- 1.79 nm when treated with a reducing agent, dithiothreitol (DTT). cPAM G3-His-Arg showed much higher transfection efficiency than native cPAM G3 and polyethylenimine (PEI, 25KD). In addition, cPAM G3-His-Arg displayed negligible toxicity, even at high polymer concentrations. Finally, confocal laser microscopy results showed that cPAM G3-His-Arg effectively internalized plasmid DNA into the cells. Therefore, we believe that cPAM G3-His-Arg could be a promising bioreducible vector for non-viral gene delivery with high gene transfection efficiency and low cytotoxicity.
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