期刊
MACROMOLECULAR RAPID COMMUNICATIONS
卷 40, 期 1, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/marc.201800390
关键词
biopolymers; calorimetry; nuclear magnetic resonance; secondary structure; silk; spectroscopy; X-ray scattering
资金
- National Science Foundation [DGE-1325256]
- Air Force Office of Scientific Research [FA9550-14-1-0015, FA9550-17-1-0333, FA9550-14-1-0014, FA9550-17-1-0282, FA9550-17-1-0409]
- FOGARTY INTERNATIONAL CENTER [D71TW010333, K01TW010282] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [U01EB014976] Funding Source: NIH RePORTER
Silk proteins are biopolymers produced by spinning organisms that have been studied extensively for applications in materials engineering, regenerative medicine, and devices due to their high tensile strength and extensibility. This remarkable combination of mechanical properties arises from their unique semi-crystalline secondary structure and block copolymer features. The secondary structure of silks is highly sensitive to processing, and can be manipulated to achieve a wide array of material profiles. Studying the secondary structure of silks is therefore critical to understanding the relationship between structure and function, the strength and stability of silk-based materials, and the natural fiber synthesis process employed by spinning organisms. However, silks present unique challenges to structural characterization due to high-molecular-weight protein chains, repetitive sequences, and heterogeneity in intra- and interchain domain sizes. Here, experimental techniques used to study the secondary structure of silks, the information attainable from these techniques, and the limitations associated with them are reviewed. Ultimately, the appropriate utilization of a suite of techniques discussed here will enable detailed characterization of silk-based materials, from studying fundamental processing-structure-function relationships to developing commercially useful quality control assessments.
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