A Generic Strategy for Co-Presentation of Heparin-Binding Growth Factors Based on CVD Polymerization

A multifunctional copolymer with both aldehyde and alkyne groups is synthesized by chemical vapor deposition (CVD) for orthogonal co-immobilization of biomolecules. Surface analytical methods including FTIR and XPS are used to confirm the surface modification. Heparin-binding growth factors [basic fibroblast growth factor (bFGF) in this study] can be immobilized through interaction with heparin, which was covalently attached to the CVD surface through an aldehyde-hydrazide reaction. In parallel, an alkyneazide reaction is used to orthogonally co-immobilize an adhesion peptide as the second biomolecule.