期刊
MACROMOLECULAR RAPID COMMUNICATIONS
卷 30, 期 7, 页码 498-503出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/marc.200800727
关键词
conjugated polymers; fluorescence; mercury (II); nanoparticles; sensors; silicas
资金
- National University of Singapore [ARF R-279-000-197-112/133, 8-279-000233-123, YIA R-279-000-234-123]
- Singapore Ministry of Educatio [R-279-000-255-112]
An amplified fluorescence turn-on assay for mercury(II) detection and quantification was developed. This method makes use of specific thymine/mercury(II)/thymine coordination to capture Fl-labeled DNA onto NP surface. Addition of a cationic conjugated polymer leads to an amplified Fl signal in solution. A sigmoidal Hg2+ working curve is obtained at fixed [NP] with a detection limit of 0.1 x 10(-6) M. However, by reducing [Hg2+] and [NP] simultaneously, while maintaining [Hg2+]:[DNA duplex] = 3:1, a linear calibration curve is observed with a detection limit of 5 x 10(-9) M. The CCP-assisted mercury(II) assay shows potential applications in environmental mercury detection and for industrial process control.
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