期刊
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
卷 29, 期 17, 页码 1585-1595出版社
WILEY-BLACKWELL
DOI: 10.1002/rcm.7265
关键词
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资金
- Grant Agency of Masaryk University [MUNI/M/0041/2013, MUNI/A/1558/2014, MUNI/A/1014/2013]
- HistoPARK - Centre for Analysis and Modeling of Tissues and Organs from European Regional Development Fund [CZ.1.07/2.3.00/20.0185]
- Faculty of Medicine, Masaryk University
RATIONALE: Many kinds of nanoparticles (NPs) have been used for mass spectrometry (MS) so far. Here we report the first use of flower-like gold nanoparticles (AuNPs) as a mediator to enhance ionization in MS of peptides and proteins. METHODS: Flower-like AuNPs were characterized using transmission and scanning electron microscopy, UV-VIS spectrophotometry, and laser desorption/ionization (LDI)-MS and compared with polyhedral AuNPs. Mass spectra were obtained in positive ion mode using a time-of-flight (TOF) analyzer coupled with either matrix-assisted laser desorption/ionization (MALDI) or surface-assisted laser desorption/ionization (SALDI) methods. RESULTS: The intensities of peptide peaks (m/z 500-3500) were up to 7.5x and up to 7x higher using flower-like AuNPs and flower-like AuNPs-enriched alpha-cyano-4-hydroxycinnamic acid (CHCA) matrix respectively, than the classical CHCA matrix. The signals of higher mass peptide/protein peaks (m/z 3600-17000) were up to 2x higher with using flower-like AuNPs-enriched CHCA matrix than conventional CHCA matrix. The signal of profile peaks generated by intact cell MALDI-TOFMS of fibroblast suspension (m/z 4000-20000) was 2x higher with using flower-like AuNPs combined with sinapinic acid (SA) compared to SA matrix alone. The use of flower-like AuNPs as internal calibration standard for the calibration of MS spectra of peptides was performed. CONCLUSIONS: Flower-like AuNPs and flower-like AuNPs combined with CHCA or SA as combined matrices for MS measurement of peptides and proteins were used. Comparison of the conventional MALDI method and our method with flower-like AuNPs was carried out. In addition, gold clusters generated from flower-like AuNPs by SALDI provide a suitable internal calibration standard for MS analysis of peptides. Copyright (C) 2015 John Wiley & Sons, Ltd.
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