期刊
MACROMOLECULAR BIOSCIENCE
卷 14, 期 6, 页码 759-771出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.201300567
关键词
alginate hydrogels; encapsulation; self-assembly; tissue engineering
资金
- FEDER funds through the Programa Operacional Factores de Competitividade (COMPETE)
- Portuguese funds through Fundacao para a Ciencia e a Tecnologia (FCT) [Pest-C/SAU/LA0002/2011, PTDC/SAU-BEB/101235/2008, FCOMP-01-0124-FEDER-010915]
- FCT-POPH-FSE
- FCT [SFRH/BPD/85651/2012]
- INL-International Iberian Nanotechnology Laboratory
- Fundação para a Ciência e a Tecnologia [PTDC/SAU-BEB/101235/2008] Funding Source: FCT
Cellular activities in 3D are differentially affected by several matrix-intrinsic and extrinsic factors. This study highlights the relevance of optimizing initial cell densities when establishing 3D cultures for specific applications. Independently of the entrapping density, MSCs cultured within RGD-alginate hydrogels showed steady-state levels of metabolic activity and were in a nearly non-proliferative state, but recovered normal'' activity levels when retrieved from 3D matrices and re-cultured as monolayers. Importantly, high-densities promoted the establishment of cell-cell contacts with formation of multicellular clusters stabilized by endogenous ECM, and also stimulated MSCs osteogenic differentiation. These MSC-ECM microtissues may be used as building blocks for tissue engineering.
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