期刊
MACROMOLECULAR BIOSCIENCE
卷 13, 期 9, 页码 1204-1212出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.201300140
关键词
fluorescence; nanoparticles; PLGA; stem cells
The synthesis and characterization of new biodegradable polymeric NPs loaded with bovine serum albumin marked with fluorescein isothiocyanate (FITC-BSA) is reported. The protein is encapsulated in poly(D,L-lactide-co-glycolide) (PLGA) NPs by the double emulsion method with subsequent solvent evaporation. The NPs display a spherical shape with a narrow size distribution and no aggregation is observed after drying. Steady-state and time-resolved fluorescence measurements appear to be a sensitive method to investigate the protein environment on the nanometer-scale. Finally, FITC-BSA-loaded NPs are rapidly internalized in stem cells. Interestingly, 25% cells were slightly positive after 28 days.
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