期刊
MACROMOLECULAR BIOSCIENCE
卷 14, 期 3, 页码 411-418出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.201300362
关键词
cytokine release; gene expression; human primary macrophages; peptides; PVDF
资金
- DFG [GRK1035]
- IZKF BIOMAT within the medical faculty of the RWTH Aachen
- START program of the Medical Faculty of the RWTH Aachen University
Surface modifications of implants are frequently done using bioactive peptides. However, immune cells such as macrophages might evoke a rejection of an implant due to an undesired activation by the materials. Here, the influence of different strategies for peptide immobilization onto (poly)-vinylidene fluoride (PVDF) on inflammation and angiogenesis is studied. The inflammatory response of human primary macrophages is investigated by analyzing inflammatory cytokine expression. Surface roughness and adsorptive coupling have only minor effects on macrophage activation. Acrylic acid (AAc)-based covalent RGD-coupling leads to the most favorable cellular reaction, indicated by increased VEGF release. Chemical vapor deposition treated surfaces are inert, but additional covalent coupling of RGD induces a pronounced proinflammatory reaction. An in vivo angiogenesis study reveals that covalent coupling of RGD results in delayed but increased angiogenesis. It is concluded that for implant decoration with peptides, the substrate material has to be selected carefully to prevent inflammatory immune responses.
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